Adenovirus type 2 DNA replication. II. Termini of DNA replication
1975; American Society for Microbiology; Volume: 16; Issue: 4 Linguagem: Inglês
10.1128/jvi.16.4.767-774.1975
ISSN1098-5514
AutoresRalf Schilling, B Weingärtner, E.‐L. Winnacker,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoComplete, mature adenovirus type 2 DNA molecules were isolated from virus-infected HeLa cells, pulse-labeled at 20 h postinfection in [3H]thymidine pulses shorter than the time necessary for one round of viral DNA replication. After digestion with the restriction endonucleases Eco RI, Hpa I, and Hind III, a temporal order of synthesis of different regions of the viral genome was established from the relative specific radioactivities in the restriction enzyme fragments. A comparison with the physical order of these fragments revealed the existence of two termini of DNA replication towards both the molecular right and left ends, respectively, of the viral chromosome.
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