Vaccinia Virus Replication in Enucleate BSC-1 Cells: Particle Production and Synthesis of Viral DNA and Proteins
1974; American Society for Microbiology; Volume: 13; Issue: 2 Linguagem: Inglês
10.1128/jvi.13.2.488-493.1974
ISSN1098-5514
AutoresT. H. Pennington, E. A. C. Follett,
Tópico(s)Poxvirus research and outbreaks
ResumoThe growth of vaccinia virus in monolayers of BSC-1 cells enucleated by centrifugation in the presence of cytochalasin B has been studied. No evidence for the production of infectious virus in these cells was obtained, and the production of virus particles was reduced to 8.3% compared with the yield from cytochalasin-treated, uncentrifuged cells. Virus DNA and early and late polypeptides were synthesized with normal timing in enucleate cells, but in reduced amounts; cleavage of structural polypeptide precursors P4a and Px also occurred in enucleate cells. Factories containing immature virus particles were demonstrated in enucleate cells by electron microscopy; these factories were reduced in number and size compared with those found in cytochalasin-treated, uncentrifuged cells.
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