Produção Nacional
Revisado por pares
Neutrophil dysfunction induced by hyperglycemia: modulation of myeloperoxidase activity
2012; Wiley; Volume: 30; Issue: 7 Linguagem: Inglês
10.1002/cbf.2840
ISSN1099-0844
AutoresCláudia de Souza Ferreira, Tomaz Henrique Araújo, Marilene Lopes Ângelo, Paula Comune Pennacchi, Sabrina Sayori Okada, Fernanda Borges de Araújo Paula, Silene Migliorini, Maria Rita Rodrigues,
Tópico(s)Neuroinflammation and Neurodegeneration Mechanisms
ResumoOur data suggest that impaired activity of myeloperoxidase (MPO) may play an important role in the dysfunction of neutrophils from hyperglycemic rats. Neutrophil biochemical pathways include the NADPH oxidase system and the MPO enzyme. They both play important role in the killing function of neutrophils. The effect of hyperglycemia on the activity of these enzymes and the consequences with regard to Candida albicans phagocytosis and the microbicidal property of rat peritoneal neutrophils is evaluated here. The NADPH oxidase system activity was measured using chemiluminescence and cytochrome C reduction assays. MPO activity was measured by monitoring HOCl production, and MPO protein expression was analysed using Western blot and immunofluorescence. C. albicans phagocytosis and death were evaluated by optical microscopy using the May–Grunwald–Giemsa staining method. ROS generation kinetic was slightly delayed in the diabetic group. MPO expression levels were higher in diabetic neutrophils; however, MPO activity was decreased in these same neutrophils compared with the controls. C. albicans phagocytosis and killing were lower in the diabetic neutrophils. Based on our experimental model, the phagocytic and killing functions of neutrophil phagocytosis are impaired in diabetic rats because of the decreased production of HOCl, highlighting the importance of MPO in the microbicidal function of neutrophils. Copyright © 2012 John Wiley & Sons, Ltd.
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