Produção Nacional
Revisado por pares
Single-Tube Nested PCR Using Immobilized Internal Primers
2002; Future Science Ltd; Volume: 33; Issue: 6 Linguagem: Inglês
10.2144/02336bm05
ISSN1940-9818
AutoresFrederico G. C. Abath, Fábio Lopes de Melo, Roberto Pereira Werkhäuser, Lílian Maria Lapa Montenegro, Rosana Montenegro, Haiana Charifker Schindler,
Tópico(s)Biosensors and Analytical Detection
ResumoBioTechniquesVol. 33, No. 6 BenchmarksOpen AccessSingle-Tube Nested PCR Using Immobilized Internal PrimersF.G.C. Abath, F.L. Melo, R.P. Werkhauser, L. Montenegro, R. Montenegro & H.C. SchindlerF.G.C. Abath*Address correspondence to Dr. Frederico G.C. Abath, Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Av. Moraes Rego s/n, Cidade Universitária, 50670-420, Recife, Brazil. e-mail: E-mail Address: fabath@cpqam.fiocruz.brCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, Brazil, F.L. MeloCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, Brazil, R.P. WerkhauserCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, Brazil, L. MontenegroCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, Brazil, R. MontenegroCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, Brazil & H.C. SchindlerCentro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, BrazilPublished Online:17 Sep 2018https://doi.org/10.2144/02336bm05AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByOptimization of single-tube nested PCR for the detection of Echinococcus spp.Experimental Parasitology, Vol. 33Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis7 June 2021 | Clinical Rheumatology, Vol. 16Improving sensitivity of single tube nested PCR to detect fastidious microorganismsHeliyon, Vol. 6, No. 1Rapid detection of Mycobacterium tuberculosis in children using blood and urine specimens1 January 2020 | Revista da Sociedade Brasileira de Medicina Tropical, Vol. 53A highly sensitive modified nested PCR to enhance case detection in leishmaniasis15 July 2019 | BMC Infectious Diseases, Vol. 19, No. 1Sensitive and quantitative method to evaluate DNA methylation of the positive regulatory domains (PRDI, PRDII) and cAMP response element (CRE) in human endothelial nitric oxide synthase promoterNitric Oxide, Vol. 92Label-free nanostructured biosensor for Schistosoma mansoni detection in complex biological fluidsTalanta, Vol. 204Does physical exercise influence in the development of neuroeschistosomiasis?Brain Research Bulletin, Vol. 152Impedimetric nanostructured genosensor for detection of schistosomiasis in cerebrospinal fluid and serum samplesJournal of Pharmaceutical and Biomedical Analysis, Vol. 137Evaluating the methylation status of CXCR4 promoter: A cost-effective and sensitive two-step PCR methodAnalytical Biochemistry, Vol. 519Nested polymerase chain reaction in cerebrospinal fluid for diagnosing spinal cord schistosomiasis: A promising methodJournal of the Neurological Sciences, Vol. 366Single-tube nested reverse transcription-polymerase chain reaction for simultaneous detection of genotyping of porcine reproductive and respiratory syndrome virus without DNA carryover contaminationKorean Journal of Veterinary Service, Vol. 39, No. 2Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urineRevista da Sociedade Brasileira de Medicina Tropical, Vol. 48, No. 6Development and Evaluation of Single-tube Nested PCR (STNPCR) for the Detection of Porcine Circovirus type 2 (PCV2)18 October 2012 | Transboundary and Emerging Diseases, Vol. 61, No. 3An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection9 October 2013 | Revista da Sociedade Brasileira de Medicina Tropical, Vol. 46, No. 6Casos autoctones de esquistossomose mansonica em criancas de Recife, PERevista de Saúde Pública, Vol. 47, No. 4Optimization of single-tube nested PCR for the diagnosis of visceral leishmaniasisExperimental Parasitology, Vol. 134, No. 2Plague Diagnosis STNPCR and MPCR Kits Assembly, Reaction Reproducibility, and Reagent Stability25 May 2012Current epidemiological status of schistosomiasis in the state of Pernambuco, BrazilMemórias do Instituto Oswaldo Cruz, Vol. 105, No. 4Development of a multiplex single-tube nested PCR (MSTNPCR) assay for Vibrio cholerae O1 detectionJournal of Microbiological Methods, Vol. 72, No. 2Single-tube nested PCR using immobilized internal primers for the identification of dengue virus serotypesJournal of Virological Methods, Vol. 145, No. 1Development and Evaluation of a Single Tube Nested PCR Based Approach (STNPCR) for the Diagnosis of PlagueDevelopment of molecular approaches for the identification of transmission sites of schistosomiasisTransactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 100, No. 11PCR Detection of Lizard Malaria Parasites: Prevalence of Plasmodium Infections with Low-Level Parasitemia Differs by Site and SeasonJournal of Parasitology, Vol. 91, No. 6Development and Evaluation of a PCR Based Assay for Detection of the Toxic Dinoflagellate, Gymnodinium catenatum(Graham) in Ballast Water and Environmental SamplesBiological Invasions, Vol. 7, No. 6Development of a single tube hemi-nested PCR for genus-specific detection of Plasmodium in oligoparasitemic patientsTransactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 98, No. 10 Vol. 33, No. 6 Follow us on social media for the latest updates Metrics Downloaded 460 times History Published online 17 September 2018 Published in print December 2002 Information© 2018 Author(s)PDF download
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