Temperate bacteriophages collected by outer membrane vesicles in Komagataeibacter intermedius
2015; Wiley; Volume: 55; Issue: 4 Linguagem: Inglês
10.1002/jobm.201400711
ISSN1521-4028
AutoresAlla Kharina, Olga Podolich, Yuliia Faidiuk, Sergiy Zaika, A. Haidak, Olga Kukharenko, Iryna Zaets, Ф.І. Товкач, Oleg N. Reva, Maxim Kremenskoy, Natalia Kozyrovska,
Tópico(s)Microbial infections and disease research
ResumoJournal of Basic MicrobiologyVolume 55, Issue 4 p. 509-513 Short Communication Temperate bacteriophages collected by outer membrane vesicles in Komagataeibacter intermedius Alla Kharina, Corresponding Author Alla Kharina Institute of Biology of Taras Shevchenko National University of Kyiv, Kyiv, Ukraine Correspondence: Alla Kharina Alla, Department of Virology, ESC "Institute of Biology" Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv, Ukraine, 01601 E-mail:[email protected] Phone: 38(044)5213502 Fax: 38(044)5213502Search for more papers by this authorOlga Podolich, Olga Podolich Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorIuliia Faidiuk, Iuliia Faidiuk Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorSergiy Zaika, Sergiy Zaika Institute of Biology of Taras Shevchenko National University of Kyiv, Kyiv, UkraineSearch for more papers by this authorAndriy Haidak, Andriy Haidak Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorOlga Kukharenko, Olga Kukharenko Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorIryna Zaets, Iryna Zaets Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorFedor Tovkach, Fedor Tovkach Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorOleg Reva, Oleg Reva Department of Biochemistry, Bioinformatics and Computational Biology Unit, University of Pretoria, Pretoria, South AfricaSearch for more papers by this authorMaxim Kremenskoy, Maxim Kremenskoy NanoMedTech LLC, Kyiv, UkraineSearch for more papers by this authorNatalia Kozyrovska, Natalia Kozyrovska Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this author Alla Kharina, Corresponding Author Alla Kharina Institute of Biology of Taras Shevchenko National University of Kyiv, Kyiv, Ukraine Correspondence: Alla Kharina Alla, Department of Virology, ESC "Institute of Biology" Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv, Ukraine, 01601 E-mail:[email protected] Phone: 38(044)5213502 Fax: 38(044)5213502Search for more papers by this authorOlga Podolich, Olga Podolich Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorIuliia Faidiuk, Iuliia Faidiuk Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorSergiy Zaika, Sergiy Zaika Institute of Biology of Taras Shevchenko National University of Kyiv, Kyiv, UkraineSearch for more papers by this authorAndriy Haidak, Andriy Haidak Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorOlga Kukharenko, Olga Kukharenko Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorIryna Zaets, Iryna Zaets Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorFedor Tovkach, Fedor Tovkach Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this authorOleg Reva, Oleg Reva Department of Biochemistry, Bioinformatics and Computational Biology Unit, University of Pretoria, Pretoria, South AfricaSearch for more papers by this authorMaxim Kremenskoy, Maxim Kremenskoy NanoMedTech LLC, Kyiv, UkraineSearch for more papers by this authorNatalia Kozyrovska, Natalia Kozyrovska Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, UkraineSearch for more papers by this author First published: 15 January 2015 https://doi.org/10.1002/jobm.201400711Citations: 11Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced with mitomycin C and nalidixic acid. Transmission electron microscopy analysis exhibited tailed bacteriophages belonging to Myoviridae. A PCR assay targeting the capsid gene of the myoviruses proved phylogenetic position of induced phages. Nalidixic acid was poor inducer of prophages, however, it induced the OMV-like particles release. Size of OMVs depended on an antibiotic applied for phage induction and varied in the range of 30–80 and 120–200 nm. Inside some of them, tails of phages have been visible. Under conditions, inducing prophages, OMVs acted as the collectors of formed phage particles, using outer membrane receptors for phage detection (in this case, outer membrane siderophore receptor), and fulfilled therefore "a cleaning," as well as defensive functions, preventing bacteriophage spread outside population. This is the first description of myoviruses affiliated to K. intermedius, as well as outer membrane vesicles interaction with phages within this host. Citing Literature Volume55, Issue4April 2015Pages 509-513 RelatedInformation
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