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BIBTEX RIS

Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions

1993; American Society for Microbiology; Volume: 175; Issue: 2 Linguagem: Inglês

10.1128/jb.175.2.553-556.1993

ISSN

1098-5530

Autores

Dana Boyd, Beth Traxler, Jon Beckwith,

Tópico(s)

Protein Structure and Dynamics

Resumo

An approach to analyzing the topology of membrane proteins with alkaline phosphatase fusions is described. Precise fusions were constructed by using polymerase chain reaction at the C terminus of each hydrophilic region of the membrane protein. The disruption of topogenic signals is thereby minimized, and predictable anomalous results are avoided. The Escherichia coli MalG protein has been analyzed.

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