Enzymatic Analysis of Isomeric Trithymidylates Containing Ultraviolet Light-induced Cyclobutane Pyrimidine Dimers
1989; Elsevier BV; Volume: 264; Issue: 11 Linguagem: Inglês
10.1016/s0021-9258(18)83357-9
ISSN1083-351X
AutoresMichael Weinfeld, Michel Liuzzi, M. C. Paterson,
Tópico(s)Biochemical and Molecular Research
ResumoPhage T4polynucleotide kinase (EC 2.7.1.78)proved incapable of catalyzing the phosphorylation of thymidylyl-(3'+5')-thymidine containing either a cissyn-cyclobutane pyrimidine dimer (d-T T) or a 6-4'-[pyrimidin-2'-one]pyrimidine photoproduct (d-T[p]-T), and similarly the UV-modified compounds of (dT)s bearing either photoproduct at their 5'-end (d-T TpT and d-T[p]TpT).In contrast, the 3'-structural isomers of these trinucleotides (d-TpT T and d-TpT[p]T) were phosphorylated at the same rate as the parent compound.These phosphorylatable lesioncontaining oligonucleotides are quantitatively released from UV-irradiated poly(dA):poly(dT) by enzymatic hydrolysis with snake venom phosphodiesterase and alkaline phosphatase (Liuzzi, M., Weinfeld, M., and
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