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Steroid metabolism in fish

1979; Elsevier BV; Volume: 38; Issue: 2 Linguagem: Inglês

10.1016/0016-6480(79)90207-7

1095-6840

B. Truscott,

Aquaculture Nutrition and Growth

Intraarterial injection of [3H]cortisol in the trout Salmo gairdnerii resulted in accumulation of radioactive catabolites in the gall bladder bile. The catabolites were present mainly as water-soluble conjugates of polar derivatives of cortisol. The steroid moieties, released by β-glucuronidase and aryl sulfatase, of the conjugates, were identified, in order of their quantitative importance as tetrahydrocortisone, 20β-cortolone, tetrahydrocortisol, and 5β-dihydrocortisone, cortisol, and cortisone. The identity of the steroids indicated the action of a 4-ene-5β-reductase, 3α-hydroxysteroid dehydrogenase, 20β-hydroxysteroid dehydrogenase, and 11-hydroxysteroid dehydrogenase. There was no evidence of 5α-reduction or oxidative cleavage of the sidechain.