Silica gel thin-layer chromatography of acidic phospholipids
1974; Elsevier BV; Volume: 89; Issue: 2 Linguagem: Inglês
10.1016/s0021-9673(01)99402-6
ISSN1873-3778
Autores Tópico(s)Lipid metabolism and biosynthesis
ResumoAbstract The monovalent and divalent salt forms of two acidic phospholipids (cardiolipin and phosphatidylinositol) have been subjected to thin-layer chromatography on two different adsorbents commonly used for the chromatography of lipids. It was found that in some thin-layer chromatographic systems, an acidic phospholipid displays different chromatographic behaviour when applied to the plate as its monovalent salt and when applied as its divalent salt. The results show that misleading results will often occur in the thin-layer chromatography of acidic phospholipids unless the ionic states of both the phospholipid and the adsorbent are properly appreciated.
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