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Effect of culture volume and adult density on the neonate production ofDaphnia magna, as a test organism for aquatic toxicity tests

2000; Wiley; Volume: 15; Issue: 3 Linguagem: Inglês

10.1002/1522-7278(2000)15

1522-7278

Fernando Mart�nez-Jer�nimo, F�lix Espinosa-Ch�vez, Rafael Villase�or,

Toxic Organic Pollutants Impact

Environmental ToxicologyVolume 15, Issue 3 p. 155-159 Effect of culture volume and adult density on the neonate production of Daphnia magna, as a test organism for aquatic toxicity tests Fernando Martínez-Jerónimo, Corresponding Author Fernando Martínez-Jerónimo [email protected] Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoLaboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this authorFélix Espinosa-Chávez, Félix Espinosa-Chávez Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this authorRafael Villaseñor, Rafael Villaseñor Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this author Fernando Martínez-Jerónimo, Corresponding Author Fernando Martínez-Jerónimo [email protected] Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoLaboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this authorFélix Espinosa-Chávez, Félix Espinosa-Chávez Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this authorRafael Villaseñor, Rafael Villaseñor Laboratorio de Acuacultura, Escuela Nacional de Ciencias Biológicas, I. P. N. Apdo. Postal: C.O.N.-432, México, D. F. 06400, MéxicoSearch for more papers by this author First published: 14 June 2000 https://doi.org/10.1002/1522-7278(2000)15:3 3.0.CO;2-XCitations: 29AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Neonates of Daphnia magna are widely used for bioassays in aquatic toxicology. Although this species is considered as a world-wide reference, its low repeatability of the results in the ecotoxicological studies, are due to the differences in maintenance conditions of the brood stock. We studied the combined effects of adult parthenogenetic female density and culture volume on the total fecundity. Extrapolated densities from 5 to 40 adults L−1, in volumes from 100 to 400 mL were tested. The green microalga Scenedesmus incrassatulus was used as food (1.30E+06 cells mL−1). Once the reproduction began, the neonates produced were counted daily and discarded to avoid crowding effects. The experiments were carried out for 40 days. The maximal total offspring per female, i.e., 832 and 755 neonates, were obtained in the extrapolated densities of 5 and 10 ind. L−1, respectively, using one adult female per culture vessel (in 200 and 100 mL of medium). When the quantity of the culture medium increased, despite a lower female density, the fecundity decreased and also the total offspring per female. For optimal neonate production, the parthenogenetic females should be individually cultured in small volumes (100 or 200 mL). © 2000 John Wiley & Sons, Inc. Environ Toxicol 15: 155–159, 2000 Citing Literature Volume15, Issue32000Pages 155-159 RelatedInformation