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Interaction of pH and NaCl on Enumeration of Heat-Stressed Staphylococcus aureus

1974; American Society for Microbiology; Volume: 27; Issue: 3 Linguagem: Inglês

10.1128/am.27.3.443-447.1974

0003-6919

Lawrence R. Smolka, F.E. Nelson, Lee M. Kelley,

Antimicrobial Resistance in Staphylococcus

The effect of pH level and NaCl concentrations, alone and in combination, on the enumeration of unstressed and heat-stressed cells of three strains of Staphylococcus aureus was determined. A definite narrowing of the optimum pH range for enumeration of both unstressed and heat-stressed cells was observed as the NaCl concentration was increased from 0.0 to 7.5%. Counts of unstressed cells diminished only slightly with increases in NaCl, whereas heat-stressed cells showed a marked sensitivity to NaCl concentrations of 4% and above, regardless of the pH level. Because of this sensitivity to NaCl, recoveries were far poorer than with unstressed cells at NaCl concentrations of 4% and above.