Artigo Revisado por pares

Protein kinase inhibitor, H-7, directly affects receptor channels

1991; Elsevier BV; Volume: 124; Issue: 2 Linguagem: Inglês

10.1016/0304-3940(91)90106-4

ISSN

1872-7972

Autores

Mariano Amador, John A. Dani,

Tópico(s)

Nicotinic Acetylcholine Receptors Study

Resumo

Compounds commonly used to modify protein phosphorylation have been found to interact directly with K channels, Ca channels, and acetylcholine receptor channels. We report that a general protein kinase inhibitor, H-7, directly affects N-methyl-d-aspartate (NMDA) receptors independent of protein kinase activity. Single-channel records in the presence of H-7 have fewer bursts of activity, and the currents are interrupted in a voltage-dependent manner by brief closures. Hippocampal whole-cell currents induced by NMDA are not decreased by H-7, in part, because the single-channel currents have longer burst lengths that compensate for the brief closures.

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