Identification of a repressor gene involved in the regulation of NAD de novo biosynthesis in Salmonella typhimurium
1988; American Society for Microbiology; Volume: 170; Issue: 1 Linguagem: Inglês
10.1128/jb.170.1.117-125.1988
ISSN1098-5530
AutoresNing Zhu, Baldomero M. Olivera, J R Roth,
Tópico(s)RNA and protein synthesis mechanisms
ResumoMutations at the nadI locus affect expression of the first two genes of NAD synthesis, nadA and nadB, which are unlinked. Genetic data imply that the regulatory effects of nadI mutations are not due to indirect consequences of physiological alterations. Two types of mutations map in the nadI region. Common null mutations (nadI) show constitutive high-level expression of the nadB and nadA genes. Rare nadIs mutations cause constitutive low-level expression of nadB and nadA. Some nadIs mutations shut off the expression of the biosynthetic genes sufficiently to cause a nicotinic acid auxotrophy. Spontaneous revertants of auxotrophic nadIs mutants have a NadI- phenotype, including some with deletions of the nadI locus. The nadI locus encodes a repressor protein acting on the unlinked nadA and nadB genes.
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