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Intra- and interspecific divergence in the nuclear sequences of the clock gene period in species of the Drosophila buzzatii cluster
2010; Wiley; Volume: 48; Issue: 4 Linguagem: Inglês
10.1111/j.1439-0469.2010.00564.x
ISSN1439-0469
AutoresFernando Faria Franco, Erica Cristina Carvalho Silva-Bernardi, F. M. Sene, Esteban Hasson, Maura Helena Manfrin,
Tópico(s)Insect-Plant Interactions and Control
ResumoJournal of Zoological Systematics and Evolutionary ResearchVolume 48, Issue 4 p. 322-331 Intra- and interspecific divergence in the nuclear sequences of the clock gene period in species of the Drosophila buzzatii cluster Divergencia inter y intraespecifica en las secuencias nucleares del gene period en las especies del "cluster"Drosophila buzzatii. Fernando Faria Franco, Fernando Faria Franco Universidade Federal de São Carlos. Campus SorocabaSearch for more papers by this authorErica Cristina Carvalho Silva-Bernardi, Erica Cristina Carvalho Silva-Bernardi Departamento de Genética. Faculdade de Medicina de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this authorFabio Melo Sene, Fabio Melo Sene Departamento de Genética. Faculdade de Medicina de Ribeirão Preto. Universidade de São Paulo, Brazil Departamento de Biologia. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this authorEsteban Ruben Hasson, Esteban Ruben Hasson Departamento de Ecología, Genética y Evolución. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires, ArgentinaSearch for more papers by this authorMaura Helena Manfrin, Maura Helena Manfrin Departamento de Biologia. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this author Fernando Faria Franco, Fernando Faria Franco Universidade Federal de São Carlos. Campus SorocabaSearch for more papers by this authorErica Cristina Carvalho Silva-Bernardi, Erica Cristina Carvalho Silva-Bernardi Departamento de Genética. Faculdade de Medicina de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this authorFabio Melo Sene, Fabio Melo Sene Departamento de Genética. Faculdade de Medicina de Ribeirão Preto. Universidade de São Paulo, Brazil Departamento de Biologia. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this authorEsteban Ruben Hasson, Esteban Ruben Hasson Departamento de Ecología, Genética y Evolución. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires, ArgentinaSearch for more papers by this authorMaura Helena Manfrin, Maura Helena Manfrin Departamento de Biologia. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo, BrazilSearch for more papers by this author First published: 10 March 2010 https://doi.org/10.1111/j.1439-0469.2010.00564.xCitations: 15 Corresponding author: Fernando Faria Franco (franco@ufscar.br)Contributing authors: Erica Cristina Carvalho Silva-Bernardi (eccsilva@rge.fmrp.usp.br), Fabio Melo Sene (famesene@usp.br), Esteban Ruben Hasson (estebanhasson@yahoo.com.ar), Maura Helena Manfrin (maura@rge.fmrp.usp.br) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstracten We have measured nucleotide variation in the CLOCK/CYCLE heterodimer inhibition domain (CCID) of the clock X-linked gene period in seven species belonging to the Drosophila buzzatii cluster, namely D. buzzatii, Drosophila koepferae, Drosophila antonietae, Drosophila serido, Drosophila gouveai, Drosophila seriema and Drosophila borborema. We detected that the purifying selection is the main force driving the sequence evolution in period, in agreement with the important role of CCID in clock machinery. Our survey revealed that period provides valuable phylogenetic information that allowed to resolve phylogenetic relationships among D. gouveai, D. borborema and D. seriema, which composed a polytomic clade in preliminary studies. The analysis of patterns of intraspecific variation revealed two different lineages of period in D. koepferae, probably reflecting introgressive hybridization from D. buzzatii, in concordance with previous molecular data. Resumenes En este trabajo hemos medido la variación nucleotídica en el dominio CCID en el gen period, localizado en el cromosoma X, asociado a los ritmos circadianos, el enjambre de siete especies que conforman denominado "cluster"Drosophila buzzatii: D. buzzatii, D. koepferae, D. antonietae, D. serido, D. gouveai, D. seriema y D. borborema. Nuestro estudio mostró que la selección purificadora es la principal fuerza que gobierna la evolución de period, en acuerdo con una región que como CCID juega un papel importante en la maquinaria de los ritmos circadianos. Hemos observado que la variación en period provee información filogenética que permitió resolver la politomía que involucra a D. gouveai, D. borborema y D. seriema. Finalmente, el análisis de la variación intraespecifíca reveló la presencia de dos linajes en D. koepferae, uno de los cuales podría ser consecuencia de hibridación introgressiva desde D. buzzatii, en acuerdo con estudios anteriores en regiones génicas localizadas en otros cromosomas. Citing Literature Supporting Information Table S1. Polymorphism statistics and minimum number of recombination events applied to period gene sequences of the species of Drosophila buzzatii cluster. Table S2. Pairwise comparison of fixed differences and shared polymorphisms among the Drosophila buzzatii cluster species, where the shared polymorphism is shown in parenthesis (above diagonal). Average intraspecific (diagonal) and interspecific (below diagonal) genetic distances values were based on the Tamura and Nei model [Tamura and Nei (1993)]. Table S3. Results of Z-test of selection based on the Nei and Gojobori method (Nei and Gojobori, 1986). Figure S1. Transition (s) and transversion (v) rates plotted against divergence among sequences of the nuclear gene period based on the Tamura and Nei model (Tamura and Nei, 1993). Filename Description JZS_564_sm_tables.doc92.5 KB Supporting info item JZS_564_sm_figure.tif43.1 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume48, Issue4November 2010Pages 322-331 RelatedInformation
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