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RNA codons and protein synthesis

1966; Elsevier BV; Volume: 21; Issue: 3 Linguagem: Inglês

10.1016/0022-2836(66)90027-1

1089-8638

Fritz Rottman, Marskall Nirenberg,

RNA modifications and cancer

Substituting 5′-, 2′-, 3′-terminal or 2′-internal ribose hydroxyls of oligonucleotides markedly affected their template activity in directing the binding of AA-sRNA to ribosomes. The relative template activity of oligo U preparations was as follows: p-5′-UpUpU > UpUpU > CH3O-p-5′-UpUpU > UpUpU-3′-p > UpUpU-3′-p-OCH3 > UpUpU-2′,3′-cyclic phosphate. Trimers with (2′-5′) phosphodiester linkages, (2′-5′)-UpUpU and also (2′-5′)-ApApA, did not serve as templates for phenylalanine- or lysine-sRNA, respectively. The relative template efficiency of oligo A preparations was as follows: p-5′-ApApA > ApApA > ApApA-3′-p > ApApA-2′-p. The hexamer, ApApApApApA was considerably more active as a template than the corresponding pentamer. These data indicate that two adjacent triplets are recognized by two AA-sRNA molecules bound to nearby ribosomal sites. A doublet with 5′-terminal phosphate, pUpC, served as a template for serine-sRNA, whereas a doublet without terminal phosphate, UpC, did not. Although the template efficiency of pUpC was lower than that of the triplet UpCpU the data show that serine-sRNA can recognize pUpC.