Amino acid analysis by ion-exchange chromatography using a lithium elution gradient
1975; Elsevier BV; Volume: 115; Issue: 1 Linguagem: Inglês
10.1016/s0021-9673(00)89013-5
ISSN1873-3778
AutoresY Houpert, P Tarallo, Gérard Siest,
Tópico(s)Protein purification and stability
ResumoThe separation of amino acids has been achieved on a short column of Chromo-Beads C2 resin, with a lithium gradient-elution system. The analysis took 8 h. The separation of asparagine and glutamine from glutamic acid was highly dependent on the sample pH and on the methanol concentration in the first buffer of the gradient. The method has been applied to analysis of human plasma and granulocytes for amino acids.
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