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Enhanced DNA Extraction and PCR Amplification of Mitochondrial Genes from Formalin-Fixed Museum Specimens

1997; Future Science Ltd; Volume: 22; Issue: 3 Linguagem: Inglês

10.2144/97223bm03

1940-9818

Andrew M. Shedlock, Margo G. Haygood, Theodore W. Pietsch, Paul Bentzen,

Metabolomics and Mass Spectrometry Studies

BioTechniquesVol. 22, No. 3 BenchmarksOpen AccessEnhanced DNA Extraction and PCR Amplification of Mitochondrial Genes from Formalin-Fixed Museum SpecimensAndrew M. Shedlock, Margo G. Haygood, Theodore W. Pietsch & Paul BentzenAndrew M. Shedlock*Address correspondence to Andrew M. Shedlock, University of California at Berkeley, Department E.S.P.M., 201 Wellman Hall, Berkeley, CA 94720, USA. Internet: E-mail Address: shedlock@nature.berkeley.eduUniversity of Washington, Seattle, WA, Margo G. HaygoodUniversity of Washington, Seattle, WA, Theodore W. PietschUniversity of Washington, Seattle, WA & Paul BentzenUniversity of Washington, Seattle, WAPublished Online:7 Aug 2018https://doi.org/10.2144/97223bm03AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByUnlocking inaccessible historical genomes preserved in formalin1 October 2021 | Molecular Ecology Resources, Vol. 22, No. 6Invasive snails, parasite spillback, and potential parasite spillover drive parasitic diseases of Hippopotamus amphibius in artificial lakes of Zimbabwe20 August 2021 | BMC Biology, Vol. 19, No. 1New Data from Old SpecimensIchthyology & Herpetology, Vol. 109, No. 2Assessing DNA for fish identifications from reference collections: the good, bad and ugly shed light on formalin fixation and sequencing approaches17 February 2021 | Journal of Fish Biology, Vol. 98, No. 5Molecular Analysis of Dirofilaria repens Isolates from Eye-Care Patients in Greece11 August 2020 | Acta Parasitologica, Vol. 66, No. 1Systematics of DamselfishesIchthyology & Herpetology, Vol. 109, No. 1A DNA metabarcoding approach for recovering plankton communities from archived samples fixed in formalin17 February 2021 | PLOS ONE, Vol. 16, No. 2Taxonomic reassessment of Parasesarma (Crustacea: Brachyura: Decapoda: Sesarmidae) based on genetic and morphological comparisons, with the description of a new genus25 April 2020 | Zoological Journal of the Linnean Society, Vol. 5Evaluation of DNA degradation and establishment of a degradation analysis model for Lepidoptera specimensYao Xu, XueYu Ren, HongBin Wang, Mei Wang & GuoHong Li28 January 2020 | BioTechniques, Vol. 68, No. 3A Method for Fixation of Fish Larvae for Morphological and Genetic Studies1 November 2019 | Journal of Ichthyology, Vol. 59, No. 5A new species of Parasesarma (Decapoda: Brachyura: Sesarmidae) from northern Australian mangroves and its distinction from morphologically similar speciesZoologischer Anzeiger, Vol. 279Systematics and phylogeography of the Australasian mangrove crabs Parasesarma semperi and P. longicristatum (Decapoda : Brachyura : Sesarmidae) based on morphological and molecular dataInvertebrate Systematics, Vol. 32, No. 1Reply to Borsa (2017): Comment on 'Annotated checklist of the living sharks, batoids and chimaeras (Chondrichthyes) of the world, with a focus on biogeographical diversity by Weigmann (2016)'27 December 2016 | Journal of Fish Biology, Vol. 90, No. 4FishTrace: a genetic catalogue of European fishes31 October 2017 | Database, Vol. 2017Ancient DNA analyses of museum specimens from selected Presbytis (primate: Colobinae) based on partial Cyt b sequencesPhylogeny of Trachylepis sp. 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Chase, Ron J. Etter, Michael A. Rex & Joseph M. Quattro15 August 2018 | BioTechniques, Vol. 24, No. 2 Vol. 22, No. 3 Follow us on social media for the latest updates Metrics Downloaded 820 times History Published online 7 August 2018 Published in print March 1997 Information© 2018 Author(s)PDF download