Estudo da viabilidade celular comparando os meios de conservação para enxerto ósseo de calota craniana: análise microscópica e imunoistoquímica em ratos
2005; Linguagem: Inglês
ISSN
2639-6459
Autores Tópico(s)Oral and gingival health research
ResumoTanaka FY. Study of cellular viability comparing storage media for skull vault bone graft. A microscopic and immunohistochemical analysis in rats. [Tese]. Aracatuba: UNESP Universidade Estadual Paulista; 2005. The aim of this study was to analyze cellular viability comparing storage media for skull vault bone graft. Preservation of viable cells in bone graft procedures is of paramount importance to obtain osteogenesis. Forty-three male used in this study. After antisepsis of the operative field, a linear incision was made on the middle region of the skull vault to obtain a bone graft from the right and left parietal areas. The grafts were removed with a 5-mm diameter trephine bur coupled to low-speed handpiece under continuous irrigation with sterile 0.9% saline. The graft pieces were placed in sterile 5-mL test tubes with caps, and were properly identified according to the group and maintained inside the test tubes as per each group conditions. The storage media evaluated for preservation of graft cellular viability were 0.9% saline (Group I) and Euro Collins solution (Group II). In order to assess whether the temperature has a direct influence on the maintenance of cellular viability, the analysis was extended to bone grafts stored at room temperature (Group III) and bone grafts with no solution, but maintained in ice (Group IV). Cellular viability was evaluated by immediate histological and immunohistochemical analyses. For each group, cellular viability was analyzed at 6, 12, 24 and 30 hours after procedure. The results of this study showed that Euro Collins solution yielded better performance than 0.9% saline as regards the maintenance of bone graft cellular viability (up to 30 hours). Key-words: Bone and bones. Bone transplantation. Cell survival. Graft survival. Lista de Abreviaturas PVPI.........: Polivinilpirrolidona iodada PBS : Solucao Tampao Fosfato HE : Hematoxicilina e eosina OPG........: Osteoprotegerina (Rabbit anti-opgSanta Cruz Biotechnology) RANK......: Rabbit anti-rankSanta Cruz Biotechnology) RANKL ...: Goat anti-ranklSanta Cruz Biotechnology) DAB......: Diaminobenzidina Lista de Figuras Lista de Figuras FIGURA 1 Grupo Euro Collins 24 horas – OPG, borda da calota (original, 160x, Diaminobenzidina. 31 FIGURA 2 Grupo seco ambiente 30 horas (original, 160x, HE). 32 FIGURA 3 Grupo seco gelo 30 horas (original,) 160x, HE). 32 FIGURA 4 Grupo imediato – OPG (original, 169x, Diaminobenzidina). 33 FIGURA 5 Grupo seco gelo 6 horas – RANKL, periferia da calota (original, 63x, Diaminobenzidina). 33 FIGURA 6 Grupo seco ambiente 6 horas – RANKL (original, 63x, Diaminobenzidina) 34 FIGURA 7 Grupo soro fisiologico 6 horas – RANK (original, 160x, Diaminobenzidina). 34 FIGURA 8 Grupo Euro Collins 30 horas – OPG (original, 63x, Diaminobenzidina). 35 FIGURA 9 Grupo Euro Collins 30 horas – OPG (original, 160x, Diaminobenzidina). 35 FIGURA 10 Grupo controle da imunoistoquimica (original, 63x, Diaminobenzidina). 36 FIGURA 11 Ratos (Rattus norvegicus albinus.Wistar) acondicionados em gaiolas (Bioterio e Centro Cirurgico Experimental “Ilidio Teodoro”-UNESP. 66 FIGURA 12 Ratos machos com peso corporal variando entre 250 a 300 gramas. 66 FIGURA 13 Anestesia por via intramuscular. 66 FIGURA 14 Cloridrato de Ketamina e Cloridrato de Xilazina. 66 FIGURA 15 Tricotomia. 66 FIGURA 16 Antissepsia com PVPI. 66 FIGURA 17 Incisao com lâmina 15. 67 FIGURA 18 Descolamento do retalho mucoperiostal com descolador de Molt e Hollemback 3S. 67
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