Portal de Periódicos da CAPES

Nucleotide sequence of the self-priming 3' terminus of the single-stranded DNA extracted from the parvovirus Kilham rat virus

1979; American Society for Microbiology; Volume: 30; Issue: 3 Linguagem: Inglês

10.1128/jvi.30.3.946-950.1979

1098-5514

Lois Ann Salzman, P. Fabisch,

CRISPR and Genetic Engineering

The parvovirus genome is a linear, single-stranded DNA molecule with double-stranded hairpin termini. The 3' terminus can serve in vitro as a self-primer for the synthesis of a double-stranded viral DNA intermediate. We have sequenced the nucleotides in the 3' terminus and propose a model for the secondary structure of the terminus and the in vitro origin of replication for the complementary viral DNA strand.