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Effects of Cadmium on Antioxidant Enzyme Activities in Sugar Cane

2002; Institute of Experimental Botany of the Czech Academy of Sciences; Volume: 45; Issue: 1 Linguagem: Inglês

10.1023/a

1573-8264

Ricardo Francisco Fornazier, Renato Rodrigues Ferreira, Ângela Pierre Vitória, Sílvia Maria Guerra Molina, Peter J. Lea, Ricardo Antunes Azevedo,

Plant Micronutrient Interactions and Effects

Sugar cane (Saccharum officinarum L. cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was not altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.