High-throughput oncogene mutation detection in human cancers by mass spectrometry-based genotyping
2006; American Association for Cancer Research; Volume: 12; Linguagem: Inglês
ISSN
1557-3265
AutoresRoman K. Thomas, Alissa C. Baker, Ralph DeBiasi, Whei Feng, Thomas LaFramboise, Meng Wang, Jeffrey C. Lee, Richard Nicoletti, Charlie Hatton, Liuda Ziagra, Stacey Gabriel, W. Marston Linehan, Heidi Greulich, Mark A. Rubin, Massimo Loda, Jonathan A. Fletcher, Fred J. Kaye, Kenneth C. Anderson, George D. Demetri, Reinhard Dummer, Stephan N. Wagner, Meenhard Herlyn, William R. Sellers, Matthew Meyerson, Levi A. Garraway,
Tópico(s)Molecular Biology Techniques and Applications
ResumoA106 Introduction: Translating tumor genetic information into therapeutic benefit remains hampered by a limited knowledge of critical alterations across the spectrum of human tumors, and the inability to identify these on a large scale in the clinical setting.Experimental Procedures: To begin to address these bottlenecks, we applied high-throughput, mass spectrometry-based genotyping (Sequenom, Inc.) to identify 250 known somatic mutations across 17 oncogenes in human cancer samples and cell lines. We then applied this approach to a panel of nearly 1,000 human tumors spanning 15 histologic types.Results: The oncogene genotyping approach enabled sensitive, rapid, and accurate oncogene mutation detection at a low cost per sample. Notably, mutation detection was largely refractory to stromal contamination, detecting somatic alterations present at a frequency of
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