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Chloromethane, Methyl Donor in Veratryl Alcohol Biosynthesis in Phanerochaete chrysosporium and Other Lignin-Degrading Fungi

1990; American Society for Microbiology; Volume: 56; Issue: 11 Linguagem: Inglês

10.1128/aem.56.11.3450-3457.1990

1098-5336

David B. Harper, J. A. Buswell, James T. Kennedy, John T. G. Hamilton,

Pesticide and Herbicide Environmental Studies

Chloromethane, a gaseous natural product implicated in methylation processes in Phellinus pomaceus , has been shown to act as methyl donor in veratryl alcohol biosynthesis in the lignin-degrading fungi Phanerochaete chrysosporium, Phlebia radiata , and Coriolus versicolor , none of which released detectable amounts of CH 3 Cl during growth. When P. chrysosporium was grown in a medium containing C 2 H 3 Cl, levels of C 2 H 3 incorporation into the 3- and 4- O -methyl groups of veratryl alcohol were very high and initially similar to those observed when the medium was supplemented with l -[ methyl - 2 H 3 ]methionine. When C 2 H 3 Cl was added to cultures actively synthesizing veratryl alcohol, incorporation of C 2 H 3 was very rapid, with 81% of veratryl alcohol labeled after 12 h. By contrast, incorporation of C 2 H 3 from l -[ methyl - 2 H 3 ]methionine was comparatively slow, attaining 10% after 12 h. It is proposed that these lignin-degrading fungi possess a tightly channeled multienzyme system in which CH 3 Cl biosynthesis is closely coupled to CH 3 Cl utilization for methylation of veratryl alcohol precursors.