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The origin of bidirectional DNA replication in polyoma virus.

1987; Springer Nature; Volume: 6; Issue: 7 Linguagem: Inglês

10.1002/j.1460-2075.1987.tb02465.x

1460-2075

Eric A. Hendrickson, Christian Fritze, William R. Folk, Melvin L. DePamphilis,

Prion Diseases and Protein Misfolding

Research Article1 July 1987free access The origin of bidirectional DNA replication in polyoma virus. E. A. Hendrickson E. A. Hendrickson Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author C. E. Fritze C. E. Fritze Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author W. R. Folk W. R. Folk Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author M. L. DePamphilis M. L. DePamphilis Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author E. A. Hendrickson E. A. Hendrickson Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author C. E. Fritze C. E. Fritze Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author W. R. Folk W. R. Folk Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author M. L. DePamphilis M. L. DePamphilis Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. Search for more papers by this author Author Information E. A. Hendrickson1, C. E. Fritze1, W. R. Folk1 and M. L. DePamphilis1 1Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115. The EMBO Journal (1987)6:2011-2018https://doi.org/10.1002/j.1460-2075.1987.tb02465.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The nucleotide locations of RNA-p-DNA covalent linkages in polyoma virus (PyV) replicating DNA were mapped in the region containing the genetically required origin of DNA replication (ori). These linkages mark the initiation sites for RNA-primed DNA synthesis. A clear transition was identified between the presence of these linkages (discontinuous DNA synthesis) and their absence (continuous DNA synthesis) on each strand of ori. This demonstrated that PyV DNA replication, like simian virus 40 (SV40), is semi-discontinuous, and thus revealed the location of the origin of bidirectional DNA replication (OBR). The transition site on the template encoding PyV late mRNA occurred at the junction of ori-core and T-antigen binding site A. This was essentially the same site as previously observed in SV40 (Hay and DePamphilis, 1982). However, in contrast to SV40, the transition site on the template encoding PyV early mRNA was displaced towards the late gene side of ori. This resulted in a 16 nucleotide gap within ori in which no RNA-p-DNA linkages were observed on either strand. A model for the initiation of PyV DNA replication is presented. Previous ArticleNext Article Volume 6Issue 71 July 1987In this issue RelatedDetailsLoading ...