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Cloning and Expression of the cDNA for Canine Interleukin-12

1997; Mary Ann Liebert, Inc.; Volume: 17; Issue: 11 Linguagem: Inglês

10.1089/jir.1997.17.713

1557-7465

Fumiyoshi Okano, Masahiro Satoh, Katsushige Yamada,

Immune Cell Function and Interaction

We cloned the canine interleukin-12 (IL-12) subunit cDNA. Canine IL-12 exhibited sequence homology to the known sequences of human, mouse, and bovine genes at nucleotide and amino acid levels. Cotransfection of the p35 and p40 subunits of canine IL-12 cDNA clones into COS-1 cells resulted in the secretion of IL-12, which supported proliferation of the stimulated canine lymphocytes, promoted induction of canine interferon-gamma (IFN-gamma) from canine lymphocytes, and showed antitumor effect in vitro. The cloned canine IL-12 will be useful for canine therapeutic applications.