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Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus

1976; American Society for Microbiology; Volume: 127; Issue: 2 Linguagem: Inglês

10.1128/jb.127.2.829-831.1976

1098-5530

M G Bramucci, Susan T. Lovett,

Bacterial Genetics and Biotechnology

Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred at less than 0.1% the frequency of transductants for a chromosome marker. Possible reasons for the extremely reduced capacity of PBS1 to generate plasmid transductants are discussed.