Evaluation and optimization of the DNA filter assay for direct detection of enterotoxigenic Escherichia coli in the presence of stool coliforms
1986; American Society for Microbiology; Volume: 24; Issue: 1 Linguagem: Inglês
10.1128/jcm.24.1.149-151.1986
ISSN1098-660X
AutoresW C Yam, Maria Li Lung, Mun Hon Ng,
Tópico(s)Antibiotic Resistance in Bacteria
ResumoDirect detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.
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