Rapid Identification of Clinically Relevant Nocardia Species to Genus Level by 16S rRNA Gene PCR
1999; American Society for Microbiology; Volume: 37; Issue: 1 Linguagem: Inglês
10.1128/jcm.37.1.99-102.1999
ISSN1098-660X
AutoresFrédéric Laurent, F. Provost, Patrick Boiron,
Tópico(s)Mycobacterium research and diagnosis
ResumoABSTRACT Two regions of the gene coding for 16S rRNA in Nocardia species were selected as genus-specific primer sequences for a PCR assay. The PCR protocol was tested with 60 strains of clinically relevant Nocardia isolates and type strains. It gave positive results for all strains tested. Conversely, the PCR assay was negative for all tested species belonging to the most closely related genera, including Dietzia , Gordona , Mycobacterium , Rhodococcus , Streptomyces , and Tsukamurella . Besides, unlike the latter group of isolates, all Nocardia strains exhibited one Mln I recognition site but no Sac I restriction site. This assay offers a specific and rapid alternative to chemotaxonomic methods for the identification of Nocardia spp. isolated from pathogenic samples.
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