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Modulation of primary cell function of host P seudomonas bacteria by the conjugative plasmid pCAR 1

2014; Wiley; Volume: 17; Issue: 1 Linguagem: Inglês

10.1111/1462-2920.12515

1462-2920

Yurika Takahashi, Masaki Shintani, Noriyuki Takase, Yuka Kazo, Fujio Kawamura, Hirofumi Hara, Hiromi Nishida, Kazunori Okada, Hisakazu Yamane, Hideaki Nojiri,

Vibrio bacteria research studies

Summary The impacts of plasmid carriage on the host cell were comprehensively analysed using the conjugative plasmid pCAR 1 in three different P seudomonas hosts, P . putida KT 2440, P . aeruginosa PAO 1 and P . fluorescens Pf 0‐1. Plasmid carriage reduced host fitness, swimming motility, and resistance to osmotic or pH stress. Plasmid carriage brought about alterations in primary metabolic capacities in the TCA cycle of the hosts. Differentially transcribed genes in the three hosts associated with plasmid carriage were identified by growth phase‐dependent transcriptome analyses. Plasmid carriage commonly showed a greater effect on the host transcriptome at the transition and early stationary phases. The transcriptome alterations were similar between KT 2440 and PAO 1. Transcriptions of numbers of genes encoding ribosomal proteins, F ‐type ATP ase, and RNAP core in both strains were not suppressed enough in the early stationary phase by plasmid carriage. These responses may have been responsible for the reduction in host fitness, motility and stress resistances. Host‐specific responses to plasmid carriage were transcriptional changes of genes on putative prophage or foreign DNA regions. The extents of the impacts on host phenotypes and transcriptomes were similarly greatest in KT 2440 and lowest in Pf 0‐1. These findings suggest that host cell function was actively regulated by plasmid carriage.