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Heavy Chain-Producing Variants of a Mouse Myeloma Cell Line

1975; American Association of Immunologists; Volume: 114; Issue: 2_Part_1 Linguagem: Inglês

10.4049/jimmunol.114.2_part_1.655

1550-6606

Sherie L. Morrison, Matthew D. Scharff,

Click Chemistry and Applications

Abstract Eleven variants producing only heavy (H) chains have been isolated from the P3 cell line after mutagenesis with either ICR-191 or nitrosoguanidine. The H chains produced by these variants are similar both in molecular weight and by peptide maps with the parental H chain. H chains dimerize intreacellularly, are not secreted but persist as stable cytoplasmic proteins. No evidence for the continued synthesis of light (L) chains or L chain fragments has been found in these variants. These studies show that, in the P3 cell line, the synthesis of H chains can continue in the absence of detectable L chain synthesis.