Structure of Dermatan Sulfate
1968; Elsevier BV; Volume: 243; Issue: 7 Linguagem: Inglês
10.1016/s0021-9258(18)93571-4
ISSN1083-351X
Autores Tópico(s)Glycosylation and Glycoproteins Research
ResumoAbstract The properties of dermatan sulfate, isolated from umbilical cord, have been compared with those of pig skin dermatan sulfate. Like the latter polysaccharide, umbilical cord dermatan sulfate was found to be a hybrid containing both l-iduronic and d-glucuronic acid; whereas the galactosamine units of pig skin dermatan sulfate were exclusively 4-sulfated, the umbilical cord polysaccharide contained a considerable proportion of 6-sulfated galactosamine residues. The latter components were preferentially located adjacent to d-glucuronic acid moieties, while 4-sulfated galactosamine units were prevalent in l-iduronic acid-containing sections of the molecule. Evidence was obtained, however, indicating that some of the galactosamine residues adjacent to l-iduronic acid moieties were 6-sulfated. This conclusion was based on the characterization of hybrid octasaccharides obtained after degradation with testicular hyaluronidase. These octasaccharides which all had the monosaccharide sequence shown below contained 2 chondroitin 6-sulfate disaccharide units located in the reducing and nonreducing termini, and 2 l-iduronic acid-containing internal disaccharide units which were largely 4-sulfated. Approximately one-third of the latter disaccharides, however, appeared to be sulfated in position 6. [see PDF for sequence]
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