Liquid chromatography in open-tubular columns
1983; Elsevier BV; Volume: 255; Linguagem: Inglês
10.1016/s0021-9673(01)88293-5
ISSN1873-3778
AutoresJames W. Jorgenson, E. J. Guthrie,
Tópico(s)Analytical Chemistry and Sensors
ResumoA theory is developed which shows that open-tubular columns will have well defined optimum diameters lengths if limits are placed on the available pressure and analysis time. The optimum diameters are shown to be rather insensitive to choice of operating pressure and analysis time, and lie between 1 and 3μm for a wide range of conditions. A method of etching borosilicate glass capillaries to increase surface roughness and column capacity factors is described. Octadecylsilane is chemically bonded to these roughened surfaces and columns are operated in a reversed-phase mode. Detection of solutes is with an on-column fluorescence detector.
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