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The epithelium specific cell cycle regulator 14‐3‐3sigma is required for preventing entry into mitosis following ultraviolet B

2013; Wiley; Volume: 29; Issue: 6 Linguagem: Inglês

10.1111/phpp.12071

1600-0781

Sandra Vogel, Thomas Herzinger,

Ubiquitin and proteasome pathways

Summary Background Deoxyribonucleic acid damage activates cell cycle checkpoints in order to maintain genomic stability. We assessed the role of different checkpoint genes in response to ultraviolet B irradiation. Methods Cell lines expressing a dominant negative mutant of ataxia telangiectasia and R ad3 related ( A tr) protein or overexpressing Cdc25A , cells deficient for 14‐3‐3σ, N ijmegen breakage syndrome ( N bs), or A taxia telangiectasia mutated ( A tm) were treated with ultraviolet B ( UVB ) and harvested after 12 h, 24 h, or 48 h for analysis by flow cytometry. Results Functional loss of A tm, A tr, or N bs did not result in a significant alteration of the cell cycle profile. Overexpression of Cdc25A led to a delayed arrest at the G1/S transition in response to low doses of UVB . Loss of 14‐3‐3σ, a negative cell cycle regulator and downstream target of p53, caused a transient arrest at the G2/M boundary. Conclusions Loss of 14‐3‐3σ sensitizes cells to UVB . After a transient cell cycle arrest, 14‐3‐3σ‐deficient cells die by undergoing mitotic catastrophe. Cdc25A overexpression causes a delayed arrest in response to low doses of UVB . After higher doses, Cdc25A is no longer able to overrun the checkpoint. A tm, A tr, or N bs are not essential for the checkpoint response to UVB , suggesting the existence of redundant signaling pathways.