Erythropoietin
1961; Elsevier BV; Volume: 46; Issue: 2 Linguagem: Inglês
10.1016/0006-3002(61)90751-x
ISSN1878-2434
AutoresBarry J. Campbell, Robert J. Schlueter, George Weber, W. F. White,
Tópico(s)Food Quality and Safety Studies
ResumoA variety of chemical and physical data have been determined for two glycoprotein fractions obtained in an Xe-97 chromatographic separation used as the fourth step in the purification of erythropoietin from anemic sheep plasma. The larger, biologically inert fraction was shown to be the α1-glycoprotein of sheep plasma. The smaller, biologically active fraction was also shown to be a glycoprotein, clearly differentiated in chemical properties, but not separable from the inert fraction in free electrophoresis. The highly active fraction appears not to be the true erythropoietic hormone, since a biologically inert counterpart, identical in all chemical and physical criteria applied, was isolated from normal sheep plasma.
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