Determinação da atividade antimicrobiana in vitro de extratos, frações e compostos isolados de Arrabidaea brachypoda
2012; Linguagem: Inglês
ISSN
2639-6459
Autores Tópico(s)Food Science and Nutritional Studies
ResumoPlants are an important source of biologically active natural products, many of which derive from different drugs. The great diversity found in Brazil justifies the significant growth in the use of plant products as potentially herbal. The genus Arrabidaea belongs to the family Bignoniaceae, which is mainly found in tropical regions. There are found 56 genera and about 338 species in the Brazil. Plants of this family are applied as astringents, antipyretic and treatment of rheumatism, diarrhea, cancer and bacterial infections; its properties are attributed to the chemical components among these lignans, flavonoids, triterpenes, xanthones among others. Arrabidaea brachypoda is popularly known as cervejinha do campo, cipo-una or tintureiro. In order to contribute to the evaluation of the pharmacological potential of the Cerrado Biome and propose new herbal medicine for popular use, this study aimed to determine the antimicrobial activity of hydroalcoholic extracts, fractions and compounds isolated from the plant A. brachypoda, using the microdilution technique, opposed to Staphylococcus aureus, Escherichia coli, S. setubal, Pseudomonas aeruginosa, Candida albicans and Helicobacter pylori. The hydroalcoholic extracts of root, stem and leaf showed moderate activity for P. aeruginosa and the root extract showed strong activity against S. aureus, these data justify the determination of activities Checkerboard and Time-Kill for this micro-organism. The compound G5 (rutin), isolated from root showed moderate activity against both bacteria. The extracts and fractions of A. brachypoda didn’t show activity anti-H. pylori. The root extract combined with amoxicillin showed a synergistic effect against S. aureus and its anti-S. aureus was confirmed by Time-Kill test with time of 6 to 12 hours. The present work proves the antimicrobial activity of A. brachypoda and suggests the continuity of studies for the elucidation the mechanism(s) of action of this specie and the proposed development of an herbal medicine. Key-words: Arrabidaea brachypoda; Antimicrobial Activity; Checkerboard; Microdilution; Time-kill. LISTA DE ABREVIATURAS Abs – Absorbância AMH – agar Mueller-Hinton AS – agar Sangue ASD – agar Sabouraud dextrose ATCC – American Type Culture Colection CBM – concentracao bactericida minima CFM – concentracao fungicida minima CIM – concentracao inibitoria minima CMH – caldo Mueller-Hinton CSD – caldo Sabouraud dextrose DCM – diclorometano DMSO – dimetilsulfoxido G4 – acido ursolico G5 – rutina MOPS – acido 3-[N-morfino] propanossulfonico PBS – tampao fosfato de sodio RAB-3 – 3β-esteariloxi-olean-12-eno SBF – soro bovino fetal TTC – cloreto de 2,3,5-trifeniltetrazolio UFC – Unidade Formadora de Colonia
Referência(s)