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Ultrafast sonochemical synthesis of protein-inorganic nanoflowers

2015; Dove Medical Press; Linguagem: Inglês

10.2147/ijn.s90274

1178-2013

Seong Soo A. An, Hyun Gyu Park, Moon Il Kim, Bhagwan S. Batule, Ki Soo Park,

Nanocluster Synthesis and Applications

Abstract: We developed a simple but efficient method to synthesize protein-inorganic hybrid nanostructures with a flower-like shape (nanoflowers), which relies on sonication to facilitate the synthesis of the nanoflowers. With this technique, we synthesized nanoflowers containing laccase as a model protein and copper phosphate within 5 minutes at room temperature. The resulting laccase nanoflowers yielded greatly enhanced activity, stability, and reusability, and their usefulness was successfully demonstrated by applying them in the colorimetric detection of epinephrine. The strategy developed could be used to rapidly synthesize nanoflowers for various applications in biosensor and enzyme catalysis and would expand the utilization of nanoflowers in diverse fields of biotechnology. Keywords: copper phosphate, laccase, epinephrine, hybrid nanomaterials