Chapter 2 Functional Tethered Bimolecular Lipid Membranes (tBLMs)
2006; Linguagem: Inglês
10.1016/s1554-4516(05)03002-4
AutoresIngo Köper, Stefan Schiller, Frank Giess, R. Naumann, Wolfgang Knoll,
Tópico(s)Nanopore and Nanochannel Transport Studies
ResumoBiological membranes are highly complex architectures. Their central structure consists of a lipid bilayer, which fulfills many tasks and, in particular, serves as a barrier between the inside and outside of the cell. Membrane-related processes have attracted an enormous interest, but systematic studies of such complex systems are difficult and limited to only a few examples. Here, we present two different model systems that enable the study of membrane proteins in a quasi-natural environment. First, the tethered bilayer lipid membrane, where a lipid is anchored to a surface via a hydrophilic spacer and the so-formed bilayer serves as a matrix for protein incorporation. In the inverse architecture, a protein is tethered to a surface and then embedded into a lipid bilayer. In both cases, functional studies of the proteins are possible.
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