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Fusion of yeast spheroplasts

1977; American Society for Microbiology; Volume: 130; Issue: 2 Linguagem: Inglês

10.1128/jb.130.2.946-947.1977

1098-5530

P. van Solingen, J.B. van der Plaat,

Plant and animal studies

Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.