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Molecular mass determination of low-molecular-mass heparins

1993; Elsevier BV; Volume: 644; Issue: 1 Linguagem: Inglês

10.1016/0021-9673(93)80115-o

1873-3778

Hiroaki Komatsu, Kimihiko Yoshii, S Ishimitsu, Satoshi Okada, Tomoko Takahata,

Veterinary Equine Medical Research

A high-performance gel permeation chromatographic system with on-line low-angle laser light-scattering detection (BPGPC-LALLS) was used to determine molecular masses of low-molecular-mass heparins (LMMHs). Measurements at wide and narrow collection angles were compared and the application of the HPGPC-LALLS method to small molecules, with molecular masses in the range 1000–10000, was assessed. The molecular mass averages of fractionated heparins and commercially available LNMHs were also determined by ordinary HPGPC analysis using an LMMH molecular-mass calibrant, supplied by the National Institute for Biological Standards and Control for the calibration of columns. The LALLS intensity at the routinely used narrow collection angle (θcol = 1°) about doubled at the wide collection angle (θcol = 2°) and the signal-to-noise ratio was improved. The present study thus indicates that wide collection angle measurement of light scattering allows the application of the HFGPC-LALLS method to very small biopolymers of molecular mass < 10 000.