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Fate of HL-A antigens in aging cultured human diploid cell strains

1973; Elsevier BV; Volume: 78; Issue: 2 Linguagem: Inglês

10.1016/0014-4827(73)90081-5

1090-2422

Chaim Brautbar, Michele Pellegrino, Soldano Ferrone, R. Reisfeld, R.C. Payne, Leonard Hayflick,

Hematopoietic Stem Cell Transplantation

The expression of histocompatibility antigens on cultured human fibroblasts was studied utilizing a quantitative microabsorption assay. Trypsin treatment of cultured human embryonic and adult fibroblasts did not change their capacity to absorb selected HL-A alloantisera as compared with cells harvested by scraping. The density of HL-A antigens was found to remain unchanged throughout the finite in vitro lifetime of two human embryonic diploid cell strains (WI-38 and WI-26) and ten adult skin fibroblast cultures. Cultured fibroblasts derived from skin, lung, heart, and liver of one donor showed similar quantitative expression of HL-A1, 9, W5 and W16. These experiments support the contention that the HL-A marker system is at present the only system by which human fibroblasts derived from different normal human donors can be distinguished in vitro.