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Human hypoxanthine-guanine phosphoribosyltransferase. IMP-GMP exchange stoichiometry and steady state kinetics of the reaction.

1979; Elsevier BV; Volume: 254; Issue: 20 Linguagem: Inglês

10.1016/s0021-9258(19)86698-x

1083-351X

C. Salerno, Alessandro Giacomello,

Folate and B Vitamins Research

Steady state kinetics of hypoxanthine guanine phosphoribosyltransferase-catalyzed reactions are studied. The results obtained suggest that IMP, GMP, P-Rib-PP, and pyrophosphate bind to the same enzyme form, while hypoxanthine and guanine bind to a different form. Guanine activates IMP-pyrophosphorolysis. During the reaction guanine and IMP are consumed with formation of GM