Produção Nacional
Revisado por pares
Antimicrobial effect of photodynamic therapy in carious lesions in vivo, using culture and real-time PCR methods
2015; Elsevier BV; Volume: 12; Issue: 3 Linguagem: Inglês
10.1016/j.pdpdt.2015.06.003
ISSN1873-1597
AutoresPatrícia Valente Araújo, Jeane de Fátima Correia‐Silva, Ricardo Santiago Gomez, Maria de Lourdes de Andrade Massara, María Esperanza Cortés, Luiz Thadeu de Abreu Poletto,
Tópico(s)Biosensors and Analytical Detection
ResumoThe aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) in carious lesions in vivo by culture and real-time PCR methods. Ten teeth with deep active carious lesions were selected and five portions of carious dentin were removed for each tooth. Two increments were used as control, to represent the superficial and deep dentin, respectively. Methylene blue at 100 mg/L was placed in contact with the cavity for 5 min, before being irradiated with a halogen light source for 1 min. Then, after PDT, other three portions were removed. The samples were processed in laboratory and the number of viable cfu was obtained. The real-time PCR analyses were performed in two increments of carious dentin, removed before and after PDT. The Streptococcus mutans DNA was isolated from carious dentin samples and amplification and detection of DNA were performed with real-time PCR. The cavities were then restored with glass-ionomer cement. Using conventional culture methods, the results demonstrated that viable bacteria were significantly reduced in all of the agar plates following photosensitization. No difference was found between both groups regarding S. mutans DNA quantification by real-time PCR. Although PDT may not affect the number of S. mutans DNA copies immediately after the treatment, clear reduction of the number of cfu was found. Despite its promising use for eliminating bacteria in dental caries treatment, further studies are necessary to establish an effective clinical protocol for the PDT.
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