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Tetraspanins CD9 and CD151 at the immune synapse support T‐cell integrin signaling

2014; Wiley; Volume: 44; Issue: 7 Linguagem: Inglês

10.1002/eji.201344235

1521-4141

Vera Rocha‐Perugini, José María González, Emilio Tejera, Soraya López‐Martín, Marı́a Yáñez-Mó, Francisco Sánchez‐Madrid,

T-cell and B-cell Immunology

Understanding how the immune response is activated and amplified requires detailed knowledge of the stages in the formation of the immunological synapse (IS) between T lymphocytes and antigen-presenting cells (APCs). We show that tetraspanins CD9 and CD151 congregate at the T-cell side of the IS. Silencing of CD9 or CD151 blunts the IL-2 secretion and expression of the activation marker CD69 by APC-conjugated T lymphocytes, but does not affect the accumulation of CD3 or actin to the IS, or the translocation of the microtubule-organizing center toward the T-B contact area. CD9 or CD151 silencing diminishes the relocalization of α4β1 integrin to the IS and reduces the accumulation of high-affinity β1 integrins at the cell-cell contact. These changes are accompanied by diminished phosphorylation of the integrin downstream targets FAK and ERK1/2. Our results suggest that CD9 and CD151 support integrin-mediated signaling at the IS.