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Acrosin Activity of Human Spermatozoa by Means of a Simple Gelatinolytic Technique: A Method Useful for IVF

1995; Wiley; Volume: 16; Issue: 3 Linguagem: Inglês

10.1002/j.1939-4640.1995.tb00525.x

2047-2927

Ralf Henkel, C. Müller, W. Miska, Wolf‐Bernhard Schill, J. Kleinstein, H. Gips,

Reproductive biology and impacts on aquatic species

ABSTRACT: Acrosin activity was determined using a gelatinolysis technique in 100‐μl semen aliquots of 114 patients (normozoospermia, n = 90; asthenozoospermia, n = 12; oligozoospermia, n = 10; polyzoospermia, n = 2) attending an in vitro fertilization (IVF) program. Halo diameter, halo formation rate, and a calculated acrosin activity index correlated significantly with the IVF rates ( P = 0.0054, r = 0.396; P = 0.0009, r = 0.401; and P = 0.0003, r = 0.428, respectively). In cases where the halo diameter was <10 μm and halo formation rate was <60%, all patients were subfertile or infertile, that is, they showed poor or no fertilization in vitro , respectively. The assay demonstrated a relatively low sensitivity: 25.7% for halo diameter, 37.1% for halo formation rate, and 25.7% for acrosin activity index, respectively. This might be attributed to other sperm functional aspects, such as disturbed acrosome reaction or impaired zona binding.