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Aqueous gel permeation chromatography of electrolytes and polyelectrolytes, 2. Donnan salt exclusion effect

1983; Wiley; Volume: 184; Issue: 12 Linguagem: Inglês

10.1002/macp.1983.021841218

0025-116X

Satoru Kadokura, Takeaki Miyamoto, Hiroshi Inagaki,

Chemical and Physical Properties in Aqueous Solutions

Abstract The salt exclusion of electrolytes and polyelectrolytes in aqueous GPC was investigated theoretically and experimentally, in order to elucidate the feasibility of GPC as a technique for measuring the Donnan salt exclusion parameter, Γ¯, of polyelectrolytes. Three simple salts different in the valency of the anion, i.e., sodium chloride, sodium sulfate and naphthalene‐1,3,6‐trisulfonic acid trisodium salt, were eluted with aqueous NaNO 3 solution on a Sephadex G‐10 gel column. The experimental results were analyzed in terms of the Donnan equilibrium established on the gel, and the Γ¯ value of these simple salts was evaluated. It was found that the Γ¯ value from GPC was in good agreement with the theoretical value of 1/2 for an ideal case, showing that the colligative properties of electrolytes and polyelectrolytes such as Γ¯ can be accurately investigated by means of GPC. GPC experiments on the Donnan salt exclusion were further carried out for the heparin‐NaCl system by using a Sephadex G‐25 gel column. From the experimental data, the Γ¯ value of heparin was estimated.