6-Tridecylresorcylic acid, a novel ATPase inhibitor that blocks the contractile apparatus of skeletal muscle proteins.
1984; Elsevier BV; Volume: 259; Issue: 24 Linguagem: Inglês
10.1016/s0021-9258(17)42503-8
ISSN1083-351X
AutoresMasaki Kobayashi, Akiko Kajiwara, Mayumí Takáhashi, Yasushi Ohizumi, Noboru Shoji, TSUNEMATSU TAKEMOTO,
Tópico(s)Cardiovascular Function and Risk Factors
Resumo6-Tridecylresorcylic acid (TRA) isolated from a primula Lysirnachia japonica Thunb.inhibited contraction of myofibrils, superprecipitation of myosin B, and ATPase activities of myosin and actomyosin prepared from rabbit skeletal muscle in a dose-dependent manner.The ICso values in molarity of TRA were as follows: myosin (K',EDTA)-ATPase, 3.5 X lo-*; myosin Ca2'-ATPase, 3.5 X lo-'; and actomyosin Mg2+-ATPase, 1.6 x lo-'.The inhibition of ATPase activity of myofibrils by TRA was virtually reversed by washing with the fresh saline solution.Kinetic analysis of inhibitory effects of TRA suggests that the inhibition of (K+,EDTA)-ATPase activity of myosin or subfragment-1 is parabolic noncompetitive.TRA had no effect on alkaline phosphatase and choline acetyltransferase activities.TRA may provide a useful chemical tool for the study of the molecular mechanisms of actin-myosin contractile systems.
Referência(s)