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Monoclonal antibodies to chick intestinal receptors for 1,25-dihydroxyvitamin D3. Interaction and effects of binding on receptor function.

1984; Elsevier BV; Volume: 259; Issue: 2 Linguagem: Inglês

10.1016/s0021-9258(17)43583-6

1083-351X

J. Wesley Pike,

Food Allergy and Anaphylaxis Research

25-dihydroxyvitamin D,; STKMED, 0.25 M sucrose, 50 mM Tris-HC1, pH 7.4, 25 mM KCI, 5 mM MgCI2, 1 mM EDTA, and 5 mM dithiothreitol; KETD, 10 mM Tris-HC1, pH 7.4, 1 mM EDTA, 5 mM ditbiothreito1, and the indicated molar concentration of KCl, e.g.KETD-0.3; and PBS, 20 mM Na,HPO,, pH 5.2, and 0.15 M NaC1; 4A57, monoclonal antibody produced by SP2/0-4A5; 9A7y, monoclonal antibody produced by SP2/0-9A'i; 8D3p, monoclonal antibody produced by SP2/0-8D3.