Portal de Periódicos da CAPES

Murine model of tuberculosis

2002; Academic Press; Linguagem: Inglês

10.1016/s0580-9517(02)32101-9

0580-9517

Alan D. Roberts, Andrea M. Cooper, John T. Belisle, Joanne Turner, Mercedes Gonzalez‐Juarrero, Ian M. Orme,

Immune Response and Inflammation

This chapter discusses the murine model of tuberculosis and focuses on the methodologies that are utilized in laboratories for the cultivation of Mycobacterium tuberculosis. In laboratories, the strains of M. tuberculosis are cultivated for use in (i) the in-vivo determination of disease host response and pathogenesis, primarily using a murine airborne infection model; (ii) the in-vivo determination of bactericidal effectiveness of novel immunotherapy, vaccination, or chemotherapeutic agents using both the murine and guinea-pig airborne infection models; and (iii) the in-vitro testing of potential chemotherapeutic agents using a murine bone marrow derived macrophage infection model. Each of these procedures requires the cultivation of both laboratory strains and newly acquired clinical isolates of M. tuberculosis. It is essential that stock cultures of M. tuberculosis used in research experiments should be standardized in terms of the culture medium and the cultural conditions (initial inocula, temperature, aeration, agitation, subculture times, and so on). The chapter discusses the handling and cultivation of mycobacteria and propagation methods for the isolation of immunologically reactive protein fractions.