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BIBTEX RIS

Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections

2015; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 110; Issue: 4 Linguagem: Inglês

10.1590/0074-02760140457

1678-8060

Gentil Arthur Lins Bentes Mendonça de Vasconcelos, Natália Maria Lanzarini, Lyana Rodrigues Pinto Lima, Pedro Paulo de Abreu Manso, Alexandre dos Santos da Silva, Sérgio da Silva e Mouta, Juliana Rodrigues Guimarães, Márcia Terezinha Baroni de Moraes, Marcelo Pelajo‐Machado, Marcelo Alves Pinto,

Hepatitis Viruses Studies and Epidemiology

An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.