Allelic Diversity of <i>MSP1</i> Gene in <i>Plasmodium falciparum</i> from Rural and Urban Areas of Gabon
2015; Korean Society for Parasitology; Volume: 53; Issue: 4 Linguagem: Inglês
10.3347/kjp.2015.53.4.413
ISSN1738-0006
AutoresDenise Patricia Mawili-Mboumba, Noé Patrick M’Bondoukwe, Elvire Adande, Marielle Karine Bouyou-Akotet,
Tópico(s)Parasites and Host Interactions
ResumoThe present study determined and compared the genetic diversity of Plasmodium falciparum strains infecting children living in 2 areas from Gabon with different malaria endemicity. Blood samples were collected from febrile children from 2008 to 2009 in 2 health centres from rural (Oyem) and urban (Owendo) areas. Genetic diversity was determined in P. falciparum isolates by analyzing the merozoite surface protein-1 (msp1) gene polymorphism using nested-PCR. Overall, 168 children with mild falciparum malaria were included. K1, Ro33, and Mad20 alleles were found in 110 (65.5%), 94 (55.9%), and 35 (20.8%) isolates, respectively, without difference according to the site (P>0.05). Allelic families' frequencies were comparable between children less than 5 years old from the 2 sites; while among the older children the proportions of Ro33 and Mad20 alleles were 1.7 to 2.0 fold higher at Oyem. Thirty-three different alleles were detected, 16 (48.5%) were common to both sites, and 10 out of the 17 specific alleles were found at Oyem. Furthermore, multiple infection carriers were frequent at Oyem (57.7% vs 42.2% at Owendo; P=0.04) where the complexity of infection was of 1.88 (±0.95) higher compared to that found at Owendo (1.55±0.75). Extended genetic diversity of P. falciparum strains infecting Gabonese symptomatic children and high multiplicity of infections were observed in rural area. Alleles common to the 2 sites were frequent; the site-specific alleles predominated in the rural area. Such distribution of the alleles should be taken into accounts when designing MSP1 or MSP2 malaria vaccine. Key words: Plasmodium falciparum, malaria, msp1 gene, diversity, endemicity, Gabon
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