Regeneration of the GTP-bound from the GDP-bound form of human and yeast ras proteins by nucleotide exchange. Stimulatory effect of organic and inorganic polyphosphates
1986; Wiley; Volume: 161; Issue: 2 Linguagem: Inglês
10.1111/j.1432-1033.1986.tb10468.x
ISSN1432-1033
AutoresEmmanuele De Vendittis, Regina Zahn, Ottavio Fasano,
Tópico(s)PI3K/AKT/mTOR signaling in cancer
ResumoThe regeneration of the GTP-bound from the GDP-bound form of purified human and yeast ras proteins occurs in vitro by a nucleotide-exchange reaction. For both human and yeast ras proteins the dissociation of the protein-bound GDP is the rate-limiting step in the presence of Mg ions. The rate of formation of the ras. GTP complex is stimulated by weak Mg2+-chelating agents like ATP and inorganic polyphosphates and, to a lesser extent, by ADP. This suggests a possible mechanism of regulation of ras-dependent pathway(s) by intracellular metabolic products.
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